SrasV1, Main, Exploration, bibRecord, 003B35

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Identifieur interne : 003B35 ( Main/Exploration ); précédent : 003B34; suivant : 003B36

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Auteurs : S. Escutenaire [Suède] ; N. Mohamed [Suède] ; M. Isaksson [Suède] ; P. Thoren [Suède] ; B. Klingeborn [Suède] ; S. Belak [Suède] ; M. Berg [Suède] ; J. Blomberg [Suède]

Source :

Archives of virology [ 0304-8608 ] ; 2007.

RBID : Pascal:07-0088920

Descripteurs français

Pascal (Inist)
- Temps réel, Réaction chaîne polymérase RT, Détection.

English descriptors

KwdEn :
- Detection, Real time, Reverse transcription polymerase chain reaction.

Abstract

Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000415
to stream PascalFrancis, to step Curation: 000574
to stream PascalFrancis, to step Checkpoint: 000344
to stream Main, to step Merge: 003C91
to stream Main, to step Curation: 003B35

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</title>
<author><name sortKey="Escutenaire, S" sort="Escutenaire, S" uniqKey="Escutenaire S" first="S." last="Escutenaire">S. Escutenaire</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mohamed, N" sort="Mohamed, N" uniqKey="Mohamed N" first="N." last="Mohamed">N. Mohamed</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<placeName><settlement type="city">Uppsala</settlement>
<region nuts="1">Svealand</region>
<region nuts="1">East Middle Sweden</region>
</placeName>
<orgName type="university">Université d'Uppsala</orgName>
</affiliation>
</author>
<author><name sortKey="Isaksson, M" sort="Isaksson, M" uniqKey="Isaksson M" first="M." last="Isaksson">M. Isaksson</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thoren, P" sort="Thoren, P" uniqKey="Thoren P" first="P." last="Thoren">P. Thoren</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Klingeborn, B" sort="Klingeborn, B" uniqKey="Klingeborn B" first="B." last="Klingeborn">B. Klingeborn</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Berg, M" sort="Berg, M" uniqKey="Berg M" first="M." last="Berg">M. Berg</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Blomberg, J" sort="Blomberg, J" uniqKey="Blomberg J" first="J." last="Blomberg">J. Blomberg</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<placeName><settlement type="city">Uppsala</settlement>
<region nuts="1">Svealand</region>
<region nuts="1">East Middle Sweden</region>
</placeName>
<orgName type="university">Université d'Uppsala</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">07-0088920</idno>
<date when="2007">2007</date>
<idno type="stanalyst">PASCAL 07-0088920 INIST</idno>
<idno type="RBID">Pascal:07-0088920</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000415</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000574</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000344</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000344</idno>
<idno type="wicri:doubleKey">0304-8608:2007:Escutenaire S:sybr:green:real</idno>
<idno type="wicri:Area/Main/Merge">003C91</idno>
<idno type="wicri:Area/Main/Curation">003B35</idno>
<idno type="wicri:Area/Main/Exploration">003B35</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses</title>
<author><name sortKey="Escutenaire, S" sort="Escutenaire, S" uniqKey="Escutenaire S" first="S." last="Escutenaire">S. Escutenaire</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mohamed, N" sort="Mohamed, N" uniqKey="Mohamed N" first="N." last="Mohamed">N. Mohamed</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<placeName><settlement type="city">Uppsala</settlement>
<region nuts="1">Svealand</region>
<region nuts="1">East Middle Sweden</region>
</placeName>
<orgName type="university">Université d'Uppsala</orgName>
</affiliation>
</author>
<author><name sortKey="Isaksson, M" sort="Isaksson, M" uniqKey="Isaksson M" first="M." last="Isaksson">M. Isaksson</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thoren, P" sort="Thoren, P" uniqKey="Thoren P" first="P." last="Thoren">P. Thoren</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Klingeborn, B" sort="Klingeborn, B" uniqKey="Klingeborn B" first="B." last="Klingeborn">B. Klingeborn</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Virology, National Veterinary Institute</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Berg, M" sort="Berg, M" uniqKey="Berg M" first="M." last="Berg">M. Berg</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biomedical Sciences and Veterinary Public Health, Section of Parasitology and Virology, Swedish University of Agricultural Sciences</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<wicri:noRegion>Uppsala</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Blomberg, J" sort="Blomberg, J" uniqKey="Blomberg J" first="J." last="Blomberg">J. Blomberg</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Medical Sciences, Section of Clinical Virology, Academic Hospital, Uppsala University</s1>
<s2>Uppsala</s2>
<s3>SWE</s3>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Suède</country>
<placeName><settlement type="city">Uppsala</settlement>
<region nuts="1">Svealand</region>
<region nuts="1">East Middle Sweden</region>
</placeName>
<orgName type="university">Université d'Uppsala</orgName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Archives of virology</title>
<title level="j" type="abbreviated">Arch. virol.</title>
<idno type="ISSN">0304-8608</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Detection</term>
<term>Real time</term>
<term>Reverse transcription polymerase chain reaction</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Temps réel</term>
<term>Réaction chaîne polymérase RT</term>
<term>Détection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.</div>
</front>
</TEI>
<affiliations><list><country><li>Suède</li>
</country>
<region><li>East Middle Sweden</li>
<li>Svealand</li>
</region>
<settlement><li>Uppsala</li>
</settlement>
<orgName><li>Université d'Uppsala</li>
</orgName>
</list>
<tree><country name="Suède"><noRegion><name sortKey="Escutenaire, S" sort="Escutenaire, S" uniqKey="Escutenaire S" first="S." last="Escutenaire">S. Escutenaire</name>
</noRegion>
<name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<name sortKey="Belak, S" sort="Belak, S" uniqKey="Belak S" first="S." last="Belak">S. Belak</name>
<name sortKey="Berg, M" sort="Berg, M" uniqKey="Berg M" first="M." last="Berg">M. Berg</name>
<name sortKey="Blomberg, J" sort="Blomberg, J" uniqKey="Blomberg J" first="J." last="Blomberg">J. Blomberg</name>
<name sortKey="Isaksson, M" sort="Isaksson, M" uniqKey="Isaksson M" first="M." last="Isaksson">M. Isaksson</name>
<name sortKey="Klingeborn, B" sort="Klingeborn, B" uniqKey="Klingeborn B" first="B." last="Klingeborn">B. Klingeborn</name>
<name sortKey="Mohamed, N" sort="Mohamed, N" uniqKey="Mohamed N" first="N." last="Mohamed">N. Mohamed</name>
<name sortKey="Thoren, P" sort="Thoren, P" uniqKey="Thoren P" first="P." last="Thoren">P. Thoren</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003B35 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003B35 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:07-0088920
   |texte=   SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri